| | Day | Time | | Opponent | Score | |
| February |
| | 27 | 4:00PM | vs. | Indiana Institute of Technology | L-3-9 | |
| | | Game 2 | vs. | Indiana Institute of Technology | W-1-0 | |
| March |
| | 6 | 11:00AM | vs. | Ohio Dominican University | L-2-6 | |
| | West Florida Tournament |
| | | Game 2 | vs. | Ohio Dominican University | W-7-3 | |
| | 7 | 3:00PM | vs. | West Florida | L-2-4 | |
| | West Florida Tournament |
| | | 5:00PM | vs. | Ohio Dominican University | W-4-1 | |
| | West Florida Tournament |
| | 8 | 9:00AM | vs. | Rockhurst University | W-3-2 | |
| | West Florida Tournament |
| | | 1:00PM | vs. | Shawnee State University | L-1-3 | |
| | West Florida Tournament |
| * | 16 | 2:00PM | vs. | Hannibal-LaGrange | RAIN | |
| * | | Game 2 | vs. | Hannibal-LaGrange | RAIN | |
| * | 18 | 2:00PM | @ | Columbia | L-0-8 (6) | |
| * | | Game 2 | @ | Columbia | L-3-7 | |
| | 19 | 12:00PM | vs. | University of St. Mary | W-8-0 | |
| | McKendree Tournament |
| | 20 | 10:00AM | vs. | William Penn University | L-1-2 | |
| | McKendree Tournament |
| * | 25 | 2:00PM | @ | Park | RAIN | |
| * | | Game 2 | @ | Park | RAIN | |
| | 31 | 1:00PM | vs. | Culver-Stockton College | W-6-2 | |
| | | Game 2 | vs. | Culver-Stockton College | W-3-1 | |
| April |
| * | 2 | 2:00PM | vs. | William Woods | L-1-4 | |
| * | | Game 2 | vs. | William Woods | L-2-8 | |
| | | 2:30PM | @ | St. Mary-of-the-Woods College | L-0-3 | |
| | | Game 2 | @ | St. Mary-of-the-Woods College | L-1-3 | |
| | 3 | 4:00PM | @ | Lindenwood University | L-1-4 | |
| | | Game 2 | @ | Lindenwood University | L-0-3 | |
| * | 5 | 2:00PM | vs. | McKendree | W-2-1 | |
| | Played at Saint Louis University |
| * | | Game 2 | vs. | McKendree | W-5-3 | |
| | Played at Saint Louis University |
| * | 7 | 2:00PM | @ | Stephens | PPD | |
| | Played at Rockbridge High School |
| * | | Game 2 | @ | Stephens | PPD | |
| | 9 | 2:00PM | vs. | University of Saint Francis | L-2-3 | |
| | Iowa Wesleyan College Tournament |
| | | 3:45PM | vs. | Trinity Christian College | W-10-5 | |
| | Iowa Wesleyan College Tournament |
| | | 5:30PM | vs. | Purdue University-North Central | W-6-5 | |
| | Iowa Wesleyan College Tournament |
| | 10 | 9:00AM | @ | Olivet Nazarene University | L-1-12 | |
| | Iowa Wesleyan College Tournament |
| | | 10:45AM | vs. | University of Saint Francis | L-1-4 | |
| | Iowa Wesleyan College Tournament |
| * | 12 | 2:00PM | @ | Williams Baptist | L-0-2 | |
| * | | Game 2 | @ | Williams Baptist | L-0-8 | |
| | (6 inning run rule) |
| * | 14 | 2:00PM | @ | Park | W-1-0 | |
| * | | Game 2 | @ | Park | L-1-12 | |
| | 16 | 12:30PM | vs. | St. Mary-of-the-Woods College | W-4-0 | |
| | | Game 2 | vs. | St. Mary-of-the-Woods College | W-6-5 | |
| | 17 | 12:00PM | @ | Washington University | L-2-4 | |
| | | Game 2 | @ | Washington University | L-1-6 | |
| * | 19 | Game 2 | vs. | Missouri Baptist | W-8-1 | |
| * | | 2:00PM | vs. | Missouri Baptist | W-8-2 | |
| | /d/2009-10/SB_Box/MBU_vs__HSSU_GM_1.mht.htm |
| | 24 | 9:00AM | vs. | Olivet Nazarene University | L-2-7 | |
| | McKendree Tournament |
| | | 3:00PM | vs. | Robert Morris College | W-4-0 | |
| | McKendree Tournament |
| * | 26 | 2:00PM | @ | Stephens | W-4-2 | |
| | Played at McKendree University |
| * | | Game 2 | @ | Stephens | W-8-1 | |
| | Played at McKendree University |
| * | 27 | 12:30PM | vs. | Hannibal-LaGrange | W-1-0 | |
| | Played at McKendree University |
| * | | Game 2 | vs. | Hannibal-LaGrange | L-0-1 (10) | |
| | Played at McKendree University |
| | 30 | 1:00PM | @ | William Woods | L-1-3 | |
| | 2010 AMC Softball Tournament Quarterfinals - Game 1 |
| | | Game 2 | @ | William Woods | L-6-7 (9) | |
| | 2010 AMC Softball Tournament Quarterfinals - Game 2 |
