| | Day | Time | | Opponent | Score | |
| February |
| | 19 | 10:00AM | vs. | Kansas Wesleyan University | W-12-4 | |
| | Lyon College Tournament |
| | | 12:00PM | @ | Lyon College | L-1-9 | |
| | Lyon College Tournament |
| | 20 | 10:00AM | vs. | Southern Nazarene University | L-6-15 | |
| | Lyon College Tournament |
| | | 12:00PM | vs. | Central Baptist College | L-1-11 | |
| | Lyon College Tournament |
| | 26 | 4:00PM | @ | Union University | L-7-14 | |
| | | Game 2 | @ | Union University | L-0-5 | |
| | 27 | 12:00PM | @ | Lambuth University | L-0-6 | |
| | | Game 2 | @ | Lambuth University | L-1-6 | |
| March |
| | 4 | 1:30PM | vs. | Culver-Stockton College | W-3-2 (8) | |
| | | Game 2 | vs. | Culver-Stockton College | L-8-9 (10) | |
| | 5 | 2:00PM | vs. | Greenville College | W-13-0 (5) | |
| | | Game 2 | vs. | Greenville College | W-14-4 (5) | |
| | 8 | 9:00AM | vs. | Waldorf College | W-8-0 (5) | |
| | | 11:00AM | vs. | University of Saint Francis | W-12-3 (5) | |
| | 9 | 1:00PM | vs. | University of Saint Francis | W-8-3 | |
| | | 5:00PM | vs. | Marian College | W-1-0 | |
| | 10 | 9:00AM | vs. | Adrian College | W-4-0 | |
| | | 11:00AM | vs. | Bluffton College | W-5-2 | |
| | 11 | 3:00PM | vs. | University of Saint Francis | RAIN | |
| | | 5:00PM | vs. | Emmanuel College | RAIN | |
| | 12 | 1:00PM | vs. | Waldorf College | RAIN | |
| | | 5:00PM | vs. | Marian College | RAIN | |
| | 17 | 2:00PM | vs. | Illinois College | W-10-2 (6) | |
| | | Game 2 | vs. | Illinois College | W-9-1 (6) | |
| | 19 | 2:00PM | vs. | MacMurray College | W-11-1 (5) | |
| | | Game 2 | vs. | MacMurray College | W-11-1 (5) | |
| * | 20 | 1:30PM | vs. | Williams Baptist | L-3-6 | |
| * | | Game 2 | vs. | Williams Baptist | W-8-2 | |
| | 27 | 2:00PM | @ | St. Mary-of-the-Woods College | W-11-5 | |
| | | Game 2 | @ | St. Mary-of-the-Woods College | L-4-7 | |
| * | 30 | 2:00PM | @ | Hannibal-LaGrange | L-3-4 | |
| * | | Game 2 | @ | Hannibal-LaGrange | W-4-1 | |
| * | 31 | 2:00PM | @ | McKendree | L-2-7 | |
| * | | Game 2 | @ | McKendree | L-0-8 | |
| April |
| | 3 | 1:00PM | vs. | Robert Morris College | RAIN | |
| | Robert Morris University Tournament (Springfield, Ill.) |
| | | 5:00PM | vs. | Knox College | RAIN | |
| | Robert Morris University Tournament (Springfield, Ill.) |
| * | 5 | 2:00PM | vs. | Stephens | W-2-1 | |
| | Game moved from March 22 to March 23 due to weather. |
| * | | Game 2 | vs. | Stephens | W-7-3 | |
| * | 7 | 2:00PM | @ | William Woods | L-1-2 | |
| * | | Game 2 | @ | William Woods | W-3-2 | |
| | 9 | 2:00PM | @ | Mid-Continent College | W-14-0 (5) | |
| | | Game 2 | @ | Mid-Continent College | W-6-4 | |
| | 10 | 12:00PM | vs. | Missouri Valley College | L-0-1 | |
| | | Game 2 | vs. | Missouri Valley College | W-7-2 | |
| * | 12 | 2:00PM | vs. | Park | W-5-1 | |
| * | | Game 2 | vs. | Park | L-1-2 | |
| * | 15 | 2:00PM | vs. | Columbia | L-0-3 | |
| * | | Game 2 | vs. | Columbia | L-2-4 | |
| | 17 | 1:30PM | vs. | St. Mary-of-the-Woods College | W-5-0 | |
| | | Game 2 | vs. | St. Mary-of-the-Woods College | W-8-0 | |
| * | 19 | Game 2 | @ | Harris-Stowe | L-1-8 | |
| * | | 2:00PM | @ | Harris-Stowe | L-2-8 | |
| | /d/2009-10/SB_Box/MBU_vs__HSSU_GM_1.mht.htm |
| | 23 | Game 2 | @ | Westminster College | RAIN | |
| | | 2:30PM | @ | Westminster College | RAIN | |
| | 24 | 12:00PM | @ | Washington University | L-1-2 (7) | |
| | | Game 2 | @ | Washington University | RAIN | |
| | Game 2 of Double Header Rained Out. |
| | 26 | 3:00PM | @ | Principia College | W-11-0 (5) | |
| | | Game 2 | @ | Principia College | W-11-0 (5) | |
| | 28 | 2:00PM | vs. | University of Illinois at Springfield | W-3-2 | |
| | | Game 2 | vs. | University of Illinois at Springfield | L-1-9 | |
| | 30 | 10:00AM | @ | Columbia | L-3-4 | |
| | 2010 AMC Softball Tournament Quarterfinals - Game 1 |
| | | 12:00PM | vs. | Columbia | L-3-4 (8) | |
| | 2010 AMC Softball Tournament Quarterfinals -- Game 2 |
